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Top 100 Most Important Biology GK Questions for RRB Exams

If you are preparing for RRB exams like RRB ALP, RRB Technician, or RRB NTPC, then Biology is an important subject that can significantly impact your overall performance and chances of success.

To improve your Bio preparation, we’ve compiled the Top 100 Most Repeated Biology Questions frequently asked in various RRB exams. This comprehensive guide not only presents essential questions but also offers insightful analyses of key Biology topics and effective study strategies to enhance your understanding and retention.

Top 100 Biology GK Questions for RRB Exam Preparation

Most Asked Biology Topics for RRB Exams

Focusing on the right Biology topics can help your preparation and maximize your study efficiency. Below is a table highlighting the Most Asked Biology Topics in RRB exams, along with the number of questions (out of the top 100 questions) typically associated with each topic. This structured approach will help you prioritize your study sessions and cover the most relevant areas effectively.

Biology Topic Number of Questions Key Areas Covered
Human Anatomy and Physiology 37 Human body systems, organs, blood pressure, excretory system, senses, temperature regulation, hormones, reproduction
Plant Biology 14 Photosynthesis, transpiration, plant structures, plant hormones, reproduction, pharmacognosy (Quinine extraction)
Cell Biology 12 Cell organelles (e.g., lysosomes, mitochondria, ribosomes), energy production, protein synthesis, waste disposal
Nutrition and Vitamins 11 Vitamins (A, B1, B3, B6, C, K, D), deficiency diseases (rickets, scurvy, pellagra, kwashiorkor), metabolism
Respiration in Plants 9 ATP and energy currency, fermentation, cellular respiration, enzyme function
Principles of Inheritance and Variation 8 Chromosomes, inheritance laws, Mendelian genetics, sex determination
Medical Biology and Health 8 Diseases (AIDS, goiter, night blindness), medical treatments (penicillin, dialysis), hemoglobin, public health issues
Reproduction and Embryology 5 Human reproduction, embryonic development (menarche, fetus formation), reproductive hormones
Ecology and Zoology 5 Parasitic organisms, marine animals, ecological roles, animal physiology
Taxonomy and Nomenclature 3 Scientific naming systems, classification of organisms, contributions by scientists (Linnaeus)
Histology 1 Study of tissues

Top 100 Biology Questions for Railway Exam Preparation

Practice Show All Answers
Q1: What is the normal systolic and diastolic (blood pressure) pressure in humans?
A. 120 mm of Hg / 80 mm of Hg
B. 160 mm of Hg / 100 mm of Hg
C. 100 mm of Hg / 60 mm of Hg
D. 140 mm of Hg / 90 mm of Hg

Q2: Which organelle of the eukaryotic cells are also called suicidal bags?
A. Golgi apparatus
B. Mitochondria
C. Lysosomes
D. Peroxisomes

Q3: Which vitamin activates proteins and calcium essential for blood clotting?
A. Vitamin K
B. Vitamin D
C. Vitamin B12
D. Vitamin C

Q4: Rickets is caused due to the deficiency of :
A. Vitamin D
B. Vitamin B6
C. Vitamin C
D. Vitamin K

Q5: ______ is a process of energy production with the help of respiratory oxygen.
A. Anaerobic respiration
B. Fermentation
C. Photosynthesis
D. Aerobic respiration

Q6: Which process in the human digestive system is similar to the emulsification of soap on dirt?
A. Absorption in the small intestine
B. Mechanical churning in the stomach
C. Bile salts breaking large fat globules into smaller ones
D. Enzymatic digestion of proteins

Q7: Growth hormone will be secreted by which of the following endocrine glands?
A. Thyroid
B. Adrenal
C. Pancreas
D. Pituitary

Q8: Which of the following contraceptive methods is useful for controlling both unwanted pregnancies and STDs?
A. Condom
B. IUD
C. Oral contraceptives
D. Sterilization

Q9: Which components are necessary for photosynthesis?
A. Oxygen, water, sunlight and chlorophyll
B. Carbon dioxide, glucose, sunlight and chlorophyll
C. Carbon dioxide, water, sunlight and chlorophyll
D. Nitrogen, water, sunlight and chlorophyll

Q10: The process of water being lost from the leaves of the plants from the pores (Stomata) is known as:
A. Evaporation
B. Respiration
C. Transpiration
D. Photosynthesis

Q11: Which cell organelle acts as a power house of the cell?
A. Mitochondria
B. Ribosome
C. Nucleus
D. Chloroplast

Q12: Which molecule is known as the ‘Energy Currency’ of the cell?
A. NADH
B. DNA
C. ATP
D. Glucose

Q13: Bone is an example of which of the following types of tissues?
A. Nervous tissue
B. Muscle tissue
C. Connective tissue
D. Epithelial tissue

Q14: Bile secreted by the liver helps in ______.
A. Absorption of proteins
B. Digestion
C. Storage of glycogen
D. Synthesis of vitamins

Q15: Which of the following sexually transmitted killer diseases is transmitted by a virus?
A. Gonorrhea
B. Syphilis
C. AIDS
D. Chlamydia

Q16: Which part of the alimentary canal carries out the complete digestion of carbohydrates, proteins, and fats?
A. Stomach
B. Large intestine
C. Small intestine
D. Esophagus

Q17: The inner lining of the small intestine has numerous finger-like projections that are called:
A. Villi
B. Cilia
C. Rugae
D. Microvilli

Q18: Which of the following circulates around the body and plugs the leaks by helping to clot the blood at the points of injury?
A. Red blood cells
B. White blood cells
C. Platelets
D. Plasma

Q19: ______ is the liquid part of the blood of which 92% is water and the remaining 8% is proteins, minerals, hormones, enzymes, and so on.
A. Lymph
B. Hemoglobin
C. Plasma
D. Serum

Q20: Involuntary actions such as breathing, salivation, vomiting, and peristalsis are controlled by the:
A. Cerebellum
B. Medulla oblongata
C. Cerebrum
D. Thalamus

Q21: Normally, in a healthy adult, the initial filtrate in the kidneys is about 180L daily. However, the volume actually excreted is only:
A. 1 litre or 2 litres a day
B. 10 litres a day
C. 50 litres a day
D. 100 litres a day

Q22: During puberty, a woman’s first menstrual flow is known as ______.
A. Ovarian follicle
B. Menarche
C. Menstrual cycle
D. Menopause

Q23: Which part of the human reproductive system helps the embryo get its nutrition from the mother’s blood?
A. Ovary
B. Placenta
C. Uterus
D. Fallopian tube

Q24: Name the plant from which the anti-malarial drug Quinine is extracted.
A. Cinchona
B. Digitalis
C. Taxus
D. Artemisia

Q25: The system of scientific name in nomenclature of organisms which we use today was introduced by which scientist?
A. Carolus Linnaeus
B. Charles Darwin
C. Gregor Mendel
D. Louis Pasteur

Q26: In which cell organelle, the first step of cellular respiration, which is the breakdown of glucose, a six-carbon molecule, into a three-carbon molecule called pyruvate takes place?
A. Cytoplasm
B. Nucleus
C. Mitochondria
D. Chloroplast

Q27: Which excretory products is stored in the old xylem of the plants?
A. Amino acids and proteins
B. Sugars and starches
C. Enzymes and hormones
D. Resins and gums

Q28: The gaseous waste product generated during photosynthesis is:
A. Carbon dioxide
B. Nitrogen
C. Oxygen
D. Hydrogen

Q29: Who discovered Penicillin?
A. Louis Pasteur
B. Robert Koch
C. Alexander Fleming
D. Marie Curie

Q30: How was the cell discovered by Robert Hooke?
A. Using a light microscope
B. Through chemical analysis
C. By culturing cells
D. By observing cork

Q31: ______ chromosomes are found in the nuclei of human cells.
A. 46 single chromosomes
B. 24 pairs of chromosomes
C. 12 pairs of chromosomes
D. 23 pairs of chromosomes

Q32: Which of the following can change sex, indicating that sex is not genetically determined?
A. Dog
B. Elephant
C. Human
D. Snail

Q33: Which is the fat-storing tissue in our body?
A. Epithelial Tissue
B. Adipose Tissue
C. Muscle Tissue
D. Connective Tissue

Q34: Which of the following are connected by Ligament?
A. Skin to muscle
B. Muscle to bone
C. Bone to bone
D. Nerve to muscle

Q35: What are the names of the three bones in the middle ear?
A. Hammer, anvil, stirrup
B. Femur, tibia, fibula
C. Radius, ulna, carpal
D. Clavicle, scapula, humerus

Q36: Which is the largest digestive gland in humans?
A. Liver
B. Stomach
C. Pancreas
D. Gallbladder

Q37: Which cranial nerve is responsible for the sense of smell?
A. Optic
B. Olfactory
C. Vagus
D. Trigeminal

Q38: Which disease is caused by the deficiency of protein in our body?
A. Rickets
B. Pellagra
C. Kwashiorkor
D. Scurvy

Q39: Which is a substance abundantly available in the sea and administered in a certain deficiency disease?
A. Calcium
B. Iodine
C. Potassium
D. Iron

Q40: Which isotope is used in the treatment of Goitre?
A. Nitrogen
B. Carbon
C. Iodine
D. Oxygen

Q41: Scurvy, the symptoms of which include bleeding gums, may be prevented by adding ______ to the diet.
A. Vitamin K
B. Vitamin B12
C. Vitamin D
D. Vitamin C

Q42: Absence of oxygen as in anaerobic fermentation by yeast cells convert pyruvate into ______.
A. Glucose + Oxygen
B. Water + Carbon dioxide
C. Lactic acid + Energy
D. Ethanol + Carbon dioxide + Energy

Q43: Which of the following pigments has a high affinity for oxygen?
A. Carotene
B. Haemoglobin
C. Melanin
D. Chlorophyll

Q44: ______ is an acquired global impairment of intellect, memory, and personality but without impairment of consciousness.
A. Dementia
B. Autism
C. Alzheimer's disease
D. Amnesia

Q45: Which receptors are located on our tongue for taste?
A. Thermoreceptors
B. Gustatory
C. Olfactory
D. Mechanoreceptors

Q46: What is the name of the longest bone found in the thigh of a human body?
A. Tibia
B. Femur
C. Fibula
D. Humerus

Q47: Which mineral is required for the formation of the hormone thyroxin?
A. Iron
B. Magnesium
C. Calcium
D. Iodine

Q48: Which hormone is produced by Islets of langerhans to control diabetes mellitus?
A. Insulin
B. Glucagon
C. Somatostatin
D. Adrenaline

Q49: Which gland produces the insulin hormone?
A. Pancreas
B. Thyroid
C. Adrenal
D. Pituitary

Q50: ______, after growth and development of organs, becomes a fetus.
A. Blastocyst
B. Gamete
C. Embryo
D. Zygote

Q51: Who gave the laws of inheritance?
A. Charles Darwin
B. Thomas Hunt Morgan
C. Jean-Baptiste Lamarck
D. Gregor Mendel

Q52: Who is known as the ‘Father of Modern Genetics’?
A. Watson and Crick
B. Barbara McClintock
C. Charles Darwin
D. Gregor Mendel

Q53: What is the branch of biology under which tissues are studied?
A. Cytology
B. Histology
C. Physiology
D. Anatomy

Q54: What is the normal temperature of a human body?
A. 100.4°F or 38° C
B. 95°F or 35° C
C. 92°F or 33° C
D. 98.6°F or 37° C

Q55: Which of the following organisms shows a parasitic nutritive strategy?
A. Spinach
B. Moss
C. Sunflower
D. Cuscuta

Q56: Which substance is present in green leaves to help in the process of photosynthesis?
A. Carotene
B. Hemoglobin
C. Melanin
D. Chlorophyll

Q57: Green colored plastids containing chlorophyll are called-
A. Chromoplast
B. Amyloplast
C. Chloroplast
D. Leucoplast

Q58: ______ are present only in plant cells.
A. Nucleus
B. Plastids
C. Ribosomes
D. Mitochondria

Q59: Complex carbohydrates which make up cell wall in plants are called-
A. Glycogen
B. Chitin
C. Starch
D. Cellulose

Q60: Which of the following transports products of photosynthesis from the leaves to the other parts of the plant?
A. Cambium
B. Xylem
C. Phloem
D. Pericycle

Q61: The concentration of which plant hormone is highest at the top of the plant and decreases as you get closer to the roots?
A. Cytokinin
B. Gibberellin
C. Auxin
D. Ethylene

Q62: Which of the following is the female reproductive part of a flower?
A. Stamen
B. Pistil
C. Petal
D. Sepal

Q63: Which reproductive parts of the flower contain the germ cells?
A. Petals and sepals
B. Pistils and sepals
C. Ovules and anthers
D. Stamens and pistils

Q64: ______ present in the center of a flower, forms the female reproductive part.
A. Carpels
B. Petals
C. Filaments
D. Sepals

Q65: Who gave the term ‘homo sapiens’?
A. Gregor Mendel
B. C. Linnaeus
C. Aristotle
D. Charles Darwin

Q66: Which is the basic unit of classification of Living Organism?
A. Kingdom
B. Genus
C. Family
D. Species

Q67: The member of which group is usually called a sponge?
A. Echinodermata
B. Cnidaria
C. Porifera
D. Mollusca

Q68: The members of phylum ______ are exclusively free-living marine animals.
A. Chordata
B. Arthropoda
C. Annelida
D. Echinodermata

Q69: Some organisms use simple food material obtained from inorganic sources in the form of carbon dioxide and water. What are these organisms known as?
A. Heterotrophs
B. Autotrophs
C. Parasites
D. Saprotrophs

Q70: Energy reserves are found in the form of chemical bonds in living beings, these chemicals of energy are called ______.
A. Lipids
B. Glucose
C. DNA
D. Adenosine triphosphate (ATP)

Q71: A unicellular organism has a definite shape, takes food in at a specific spot, and uses like cilia to move food to that spot. Identify the organism.
A. Yeast
B. Paramaecium
C. Amoeba
D. E. coli

Q72: Which of the following transports food, carbon dioxide, and nitrogenous wastes in dissolved form?
A. Hemoglobin
B. Plasma
C. Lymph
D. Interstitial fluid

Q73: How many sex chromosomes are there in a normal human being?
A. One
B. Four
C. Three
D. Two (1 pair)

Q74: Which sets of chromosomes lead to the birth of a girl child?
A. YY
B. XY
C. XX
D. X0

Q75: What is the number of chromosomes present in the gametes (egg and sperm) of human beings?
A. Four sets of chromosomes
B. Two sets of chromosomes
C. Three sets of chromosomes
D. One set of chromosomes

Q76: How many pairs of autosomes does a normal human have?
A. 23
B. 21
C. 22
D. 20

Q77: Which of the following organelles are called the Protein Factories of the cell?
A. Golgi apparatus
B. Lysosomes
C. Mitochondria
D. Ribosomes

Q78: ______ are a kind of waste disposal system of the cell. They help to keep the cell clean by digesting any foreign materials as well as worn-out cell organelles.
A. Peroxisomes
B. Lysosomes
C. Vacuoles
D. Endosomes

Q79: Pyruvate is decomposed in the presence of oxygen in ______.
A. Mitochondria
B. Nucleus
C. Cytoplasm
D. Chloroplast

Q80: When the terminal linkage in ATP is broken using water, what is the amount of energy released?
A. 10.5 KJ/mol
B. 20.5 KJ/mol
C. 40.5 KJ/mol
D. 30.5 KJ/mol

Q81: In the respiration process, complex organic compounds such as glucose are broken down to provide energy in the form of ______.
A. RNA
B. ATP
C. DNA
D. Glucose

Q82: Asexual reproduction as in the case of amoeba in which the animal reproduces by dividing into two individuals is called:
A. Sporulation
B. Binary fission
C. Fragmentation
D. Budding

Q83: A segment of DNA that provides information to a protein is called the ______ of that protein.
A. Allele
B. Genome
C. Chromosome
D. Gene

Q84: The process of conversion of sugar into alcohol is known as:
A. Glycolysis
B. Fermentation
C. Respiration
D. Photosynthesis

Q85: The eyeball is approximately spherical in shape with a diameter of about
A. 1.8 cm
B. 4.0 cm
C. 2.3 cm
D. 3.5 cm

Q86: Which cells present in the retina are sensitive to bright and normal light, and give colour sensation?
A. Rods
B. Ganglion cells
C. Cones or cone shaped cells
D. Bipolar cells

Q87: ______ is an aperture which controls light passage in the eye.
A. Iris
B. Pupil
C. Cornea
D. Lens

Q88: Which part of the human ear collects sound from the surroundings?
A. Ossicles
B. Cochlea
C. Eardrum
D. Pinna

Q89: A dialyzer, a machine used to clean a patient’s blood, it is also known as an artificial:
A. Lung
B. Liver
C. Kidney
D. Heart

Q90: Cartilage tissue is absent in which of the following organs of the body?
A. Nose
B. Trachea
C. Kidney
D. Ear

Q91: Which part of the human body is removed from nitrogenous waste like urea and uric acid in the body?
A. Kidney
B. Lungs
C. Liver
D. Skin

Q92: In human beings, excretory products in the form of soluble nitrogen compounds are removed by the kidneys.
A. Collecting ducts
B. Glomeruli
C. Bowman's capsule
D. Nephrons

Q93: Which pigment is mainly responsible for the color of our hair, skin and Eyes?
A. Chlorophyll
B. Melanin
C. Hemoglobin
D. Carotene

Q94: ______ organs are those organs which have the same basic structural design and origin but have different functions.
A. Asymmetrical
B. Homologous
C. Analogous
D. Symmetrical

Q95: Night blindness is caused by deficiency of ______.
A. Vitamin B12
B. Vitamin A
C. Vitamin D
D. Vitamin C

Q96: The Chemical name of Vitamin-B3 is:
A. Niacin
B. Riboflavin
C. Pyridoxine
D. Thiamine

Q97: Vitamin B1 is also known as ______.
A. Pantothenic acid
B. Thiamine
C. Riboflavin
D. Niacin

Q98: What is the other name of pyridoxine?
A. Vitamin B12
B. Vitamin B6
C. Vitamin C
D. Vitamin B2

Q99: Ascorbic acid is known as:
A. Vitamin K
B. Vitamin A
C. Vitamin D
D. Vitamin C

Q100: The vitamin given for the treatment of common cold is:
A. Vitamin D
B. Vitamin C
C. Vitamin E
D. Vitamin B12

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Mastering Biology is crucial for acing RRB exams and securing your desired position. By focusing on the most critical topics and diligently practicing these top 100 questions, you’ll be well-prepared to tackle the Biology section with confidence and precision. Remember to maintain a disciplined study schedule, utilize high-quality resources, and continuously evaluate your progress through mock tests and practice papers.

Good luck with your RRB exam preparation! Stay dedicated, keep practicing, and you’ll achieve your academic and career goals.

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